Leaf chemistry plays a central role in structuring phyllosphere microbiomes. Plant populations often evolve genetic differences in leaf chemistry across region due to both abiotic and biotic selection pressures, including insect herbivory. Plants in invasive populations may reassociate with native specialist insects, providing an ideal system to examine how herbivory-mediated changes in plant chemistry affect phyllosphere microbiome. Here, we conducted a common garden experiment using Ambrosia artemisiifolia populations differing in leaf chemistry and reassociation history with a specialist beetle—Ophraella communa. We found that plant populations with a longer reassociation history exhibited stronger herbivore resistance and supported phyllosphere communities with higher alpha diversity and more complex composition. These changes were associated with shifts in concentrations of plant metabolites and the expression levels of corresponding biosynthetic genes. The abundance of the fungal pathogens, Golovinomyces, decreased with increasing herbivore resistance, while Pestaliopsis showed the opposite trend. Although reassociation history was linked to population latitude, climatic and soil conditions at the sites of origin also contributed to between-population variation in leaf chemistry and phyllosphere fungal community composition. Our study suggests that genetic differences in leaf chemistry among plant populations can strongly affect herbivore resistance and phyllosphere fungal communities. The observed alignment of reassociation history, chemical traits and phyllosphere fungal communities suggests that herbivore-mediated selection may be a key driver of microbial community evolution in invasive plants.

Land tillage disturbances and nutrient enrichment profoundly alter ecosystem processes and functions. Previous studies have explored the effects of tillage disturbance and nutrient enrichment on plant communities and soil properties. However, integrated studies of the effects of tillage disturbance and nutrient enrichment on multiple below-ground ecological processes and functions are needed. Here, we conducted a field experiment in the Hulunber grassland, establishing four treatments (control, tillage disturbance (D), nutrient enrichment (NPKμ) and tillage disturbance plus nutrient enrichment (NPKμD)) to examine their influences on plant communities, soil microbial communities, and carbon mineralization. Compared with the D treatment, the NPKμD treatment increased plant community biomass through a significant 13-fold rise in annual and biennial plant biomass (P < 0.01). Both the D treatment and NPKμD treatment significantly decreased the Shannon index of plant communities (P < 0.05). Microbial network complexity increased under NPKμ treatment whereas the D treatment reduced it. Both D treatment and NPKμ treatments significantly reduced soil carbon mineralization, and NPKμ exacerbated the negative effects of tillage disturbance (P < 0.05). Partial Least Squares Path Modeling showed that plant diversity, biomass and soil properties influenced soil carbon mineralization directly and indirectly via soil bacterial and fungal communities. Our findings suggest that nutrient enrichment promotes the recovery of plant community productivity after disturbance, while the recovery of plant diversity and soil microbial community structure may require a longer period. Therefore, achieving comprehensive ecological integrity characterized by stable plant community structure and healthy soil microbial communities requires long-term dynamic monitoring and targeted management strategies.

Warm temperate forests have the large potential to sequester atmospheric carbon dioxide (CO₂), while the interannual variability (IAV) of net forest ecosystem carbon exchange (NEE) in the global carbon cycle is still not fully understood. In this study, we conducted eddy-covariance measurement to investigate the IAV of carbon fluxes and concurrent influencing factors in a warm temperate natural oak forest from 2017 to 2022. Our results showed the natural oak forest was a strong CO₂ sink with an increase of 27.79 g C m⁻² a⁻¹ in annual carbon sequestration, resulting from a larger increase in annual gross primary production (GPP) than that of annual ecosystem respiration (Re). Precipitation in spring (PPT_spring) negatively influenced annual GPP, soil water content in spring (SWC_spring) negatively influenced annual Re, while the water conditions had lesser effect on annual NEE attributing to the synchronous changes of annual GPP and annual Re. Increase of temperature in autumn (Ta_autumn) delayed the end date of the growing season, leading to the increase in annual carbon sequestration. In addition, carbon fluxes did not significantly decrease under dramatic reduction of summer precipitation, indicating that warm temperate natural oak forest had a high resistance to seasonal drought. Our study helped us to better understand the mechanisms underlying forest carbon fluxes in response to drought in the context of future climate change.

Carbon (C) quality of non-leaf litter is closely related to decomposition rate and plays a vital role in terrestrial ecosystem C sequestration. However, to date, the global patterns and influencing factors of non-leaf litter C quality remain unclear. Here, using meta-analysis method, we quantified the characteristics and driving factors of the initial C quality of non-leaf litter (bark, branch, flower, fruit, root, stem, and wood) with 996 observations collected from 279 independent publications, including the concentrations of total C, lignin, cellulose, and hemicellulose. Results showed that (1) only total C and cellulose concentrations significantly varied among different types of non-leaf litter; (2) C quality is higher (i.e., lower concentration) in bark, branch, root, stem and wood litter from angiosperms than gymnosperms, from herbaceous than woody plants, from broadleaved than coniferous trees, and from arbuscular mycorrhizal (AM) than ectomycorrhizal (ECM) plants (except for hemicellulose concentration); and (3) the impacts of different geographic features on C quality of non-leaf litter differed among different litter types, while soil properties generally exhibited strong impacts. Overall, our results clearly show the global patterns of C quality and associated influencing factors for different types of non-leaf litter, which would be helpful for a better understanding of role of non-leaf litter in terrestrial ecosystem C cycling and for the improvement of C cycling models.