Seed mass is a key trait influencing plant form and function. It reflects parental resource investment and influences seedling growth as well as the construction of root and leaf organs. However, how seed mass regulates root and leaf functional traits in legume species remains unclear. In this study, we selected 16 common herbaceous legume species growing in pots. The results showed that seed mass exhibited a U-shaped quadratic relationship with both fine root diameter and fine root biomass. Seed mass was significantly positively correlated with single leaf area, specific leaf area and stomatal length. However, it showed no significant correlation with leaf nitrogen concentration, leaf vein traits or stomatal density. Meanwhile, the relationships between seed and root traits and between seed and leaf traits in these legume species were markedly different from those reported for global non-nitrogen-fixing plants. These results indicate that seed mass may selectively regulate certain root and leaf traits. These findings provide a new perspective for understanding the formation of life history strategies in legumes and their responses and adaptations to environmental change.

Precipitation legacy effects (PLEs) profoundly alter the recovery trajectories of semi-arid grasslands under global climate change, necessitating mechanistic quantification for accurate climate risk assessment in these vulnerable ecosystems. Based on a 7-year precipitation simulation experiment followed by an in situ natural recovery study in a semi-arid sandy grassland in Inner Mongolia, China, we characterized PLEs across multiple ecological hierarchies and varying precipitation patterns using data from the final treatment year and the first post-treatment year. Our results demonstrated that vegetation traits exhibited stronger PLEs than soil physicochemical properties. The magnitude of PLEs increased with higher functionalization (composition → productivity) and finer hierarchy (community → functional group), exceeding 50% when significant. Dry PLEs were generally stronger than wet PLEs; both exhibited bidirectional (positive/negative) performance, yet consistently showed an inverse relationship between vegetation traits and trait resilience. Mechanistically, PLEs of moderate wetting and extreme drying were primarily carried by vegetation-mediated information, whereas PLEs of moderate drying and spring drought legacies were mainly carried by soil-mediated material. Specifically, functional group composition served as the key information carrier: annuals primarily carried positive dry PLEs and negative wet PLEs, while perennials carried the opposite PLEs; and the prevalent negative PLEs in community-level productivity and species diversity were specifically attributed to perennial grasses expansion after drying and annual forbs expansion after wetting. Soil available nutrients acted as the key material carrier, promoting PLEs in annuals via synergistic physicochemical pathways. Overall, both dry and wet PLEs generally impeded the vegetation recovery of the sandy grassland ecosystem, despite positive effects on certain finer-hierarchy ecosystem traits. We conclude that ignoring PLEs may lead to a severe underestimation of climate change risks in semi-arid ecosystems, particularly regarding their most sensitive components.

Soil microbial necromass carbon (MNC), a critical component of soil organic carbon (SOC), plays a vital role in the formation and stabilization of SOC. Climate warming and increased atmospheric nitrogen (N) deposition are key factors influencing carbon sequestration in grassland ecosystems. However, the impacts of warming and N deposition, as well as their interactions, on soil MNC in arid grasslands remain poorly understood. In this study, we investigated soil MNC, including fungal necromass carbon (FNC) and bacterial necromass carbon (BNC), following a continuous 16-year manipulation of warming and N addition in a desert steppe in Inner Mongolia, China. We also analyzed these parameters in conjunction with soil microbial diversity, plant coverage and soil properties. The results showed that N addition significantly increased soil MNC and FNC, as well as fungal diversity while not affecting BNC or bacterial diversity. Warming did not significantly affect MNC (including both FNC and BNC) or soil microbial diversity and there was no interactive effect between warming and N addition. Soil MNC, FNC and BNC were found to be higher in the topsoil layer than in the subsoil layer, and FNC contributed more to SOC than BNC. Notably, the increase of soil MNC and FNC under N addition was mainly mediated by increased soil N content. This study clarifies the critical role of MNC in soil carbon storage and offers an empirically grounded basis for forecasting grassland carbon changes under future global change scenarios.

The role of local plant microbiome in affecting invasive plant growth remains unclear. We examined how aboveground and belowground microbes from 25 phylogenetically distinct local plants affect the growth and endophytic community assembly of the invasive Ageratina adenophora. Our results showed that both the plant phylogenetics and the microbial community composition of local plants shaped endophytic community assembly in A. adenophora seedlings. Phylogenetic and physicochemical factors of the local plants did not explain their effects on growth of A. adenophora. Instead, growth was affected by both the microbial sources associated with local plants and the endophytes selectively enriched by A. adenophora. Aboveground microbes promoted A. adenophora growth more strongly than belowground soil microbes. On average, A. adenophora seedlings inoculated with aboveground tissues harbored a greater relative abundance of beneficial microbes and a lower relative abundance of detrimental microbes compared to those receiving soil inoculation. These results highlight that the invasive A. adenophora can selectively recruit microbial communities from local plant microbiomes to enhance its growth.

Plant trait network (PTN) has been used to elucidate plant adaptations to environmental changes. Enhanced anthropogenic nitrogen (N) deposition has had marked impacts on grassland ecosystems. A decline in atmospheric N deposition has occurred in many regions across the globe in recent decades. However, no studies have evaluated whether and how PTNs are involved in resource-use strategies of the grassland community in response to disturbance of N input. We investigated the responses of architecture and hub traits within PTN of a grassland community to cessation of N addition for 5 years after consecutive N addition for 15 years in a temperate grassland of Inner Mongolia. We found that three functional modules (nutrient utilization, water maintenance and light acquisition) operated independently under N addition. However, a 3-fold reduction in PTN modularity was found in response to cessation of N input by enhancing the integration of water maintenance and light acquisition module. Moreover, traits associated with water maintenance were strongly coupled with the nutrient-utilization traits. Cessation of N addition shifted PTN hub traits from phosphorus (P)- to N-related traits by alleviating P limitation. The traits of key drivers for ecosystem functions remained the most sensitive and robust responders to changes in soil nutrients. These traits may function as ‘meta–traits’, and drive coordinated responses of other traits to environmental variation. We applied PTN for the first time to examine multi-trait structure and functional integration, and highlighted the necessity of integration of PTN with other analyses to understand how plants shift their strategies in adaptation to nutrient enrichment.

Drought stress significantly constrains plant growth and terrestrial ecosystem productivity by disrupting the synergistic interplay between carbon (C) assimilation and nitrogen (N) cycling. This review synthesized evidence for a multi-tiered “C-N dual limitation” feedback loop under drought conditions, driven by the following key mechanistic disruptions: (1) impaired photosynthetic C assimilation due to combined stomatal and non-stomatal limitations; (2) suppressed soil N mineralization and reduced root N uptake; (3) drought-driven shifts in microbial community structure that weaken organic N mobilization and mycorrhizal nutrient transport; and (4) a metabolic tradeoff in energy allocation, whereby Adenosine Triphosphate (ATP) and Nicotinamide Adenine Dinucleotide Phosphate Hydrogen (NADPH) are diverted from growth to antioxidant defense. To unravel the complexity of this C-N imbalance, we addressed three pivotal issues: (1) to dissect the interactive regulation of physiological, metabolic, and molecular processes that reinforce the feedback loop; (2) to contrast the C-N coordination strategies across C₃, C₄, and Crassulacean Acid Metabolism (CAM) plants, linking these differences to divergent drought resilience; (3) to evaluate pathways to mitigate this limitation, ranging from the targeting of key regulatory hubs such as TOR/SnRK1 and ABA-ROS signaling, to leveraging of rhizosphere microbial ecology, and to discuss their integration into predictive models. By integrating current insights, this review presents a coherent framework for understanding plant drought resistance and proposes actionable strategies for sustainable ecosystem management in a changing climate.