Nitrogen addition alters aboveground C:N:P stoichiometry of plants but not for belowground in an Inner Mongolia grassland

doi:10.1093/jpe/rtad041

J Plant Ecol ›› 2024, Vol. 17 ›› Issue (1): rtad041.DOI: 10.1093/jpe/rtad041

• Research Article •

Nitrogen addition alters aboveground C:N:P stoichiometry of plants but not for belowground in an Inner Mongolia grassland

Ziqi Wang^1,†, Jie Wang^2,†, Honghui Wu³, Tian Yang⁴, Yixin An^1,*, Yunlong Zhang⁵, Jianlin Bian⁶, Ying Li¹, Haiyan Ren⁷, Ariuntsetseg Lkhagva⁸, Xu Wang^2,*, Qiang Yu¹

¹School of Grassland Science, Beijing Forestry University, Beijing 100083, China,
²National Hulunber Grassland Ecosystem Observation and Research Station, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China,
³Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China,
⁴Department of Biology, University of Western Ontario, London, ON, Canada,
⁵College of Grassland Science and Technology, China Agricultural University, Beijing 100083, China,
⁶College of Resource Environment and Tourism, Capital Normal University, Beijing 100048, China,
⁷College of Agro-grassland Science, Nanjing Agricultural University, Nanjing 210095, China,
⁸Department of Biology, School of Arts and Sciences, National University of Mongolia, Ulaanbaatar 14200, Mongolia

Received:2023-06-05 Revised:2023-07-16 Accepted:2023-11-18 Online:2023-12-05 Published:2024-02-01
Contact: * E-mail: anyixin@bjfu.edu.cn (Y.A.); wangxu01@caas.cn (X.W.)
About author:^†These authors contributed equally to this work.

氮添加对内蒙古草原植物地上和地下碳氮磷化学计量学特征的影响

Abstract

Abstract: Nitrogen (N) deposition exhibits significant impacts on ecosystem functions and processes. Previous studies have indicated that N addition has an impact on the stoichiometry of plant leaf C:N:P ratios. However, few studies have focused on effects of N addition on belowground systems. This study aims to examine the impact of 7 years of N addition on above- and belowground C:N:P stoichiometry at plant community level in a temperate grassland located in Inner Mongolia. A 7-year field N addition experiment was conducted, which included six treatments: Cont: control; N1: 0.4 mol·m^-2 N; N2: 0.8 mol·m^-2 N; N3: 1.6 mol·m^-2 N; N4: 2.8 mol·m^-2 N; N5: 4 mol·m^-2 N with six replicates. Above- and belowground plant biomass and C:N:P stoichiometry were measured and analyzed. Our results showed that N addition resulted in a reduction of aboveground C concentration, but an increase in aboveground N and P concentrations, with a decrease in C:N and C:P ratios and an increase in N:P ratio. Furthermore, the aboveground C, N, and P pools all exhibited an increase as a result of N addition. However, N addition did not have any significant effect on belowground C, N, P concentrations, ratios, pools, or stoichiometric characteristics in the soil layers of 0-10, 10-30, 30-50, and 50-100 cm. These results suggest that increasing levels of N deposition significantly alter the aboveground C:N:P stoichiometry at the plant community level, which may affect functions and processes in the grassland ecosystem, but have little effect on belowground C:N:P stoichiometry.

Key words: nitrogen deposition, C:N:P stoichiometry, grassland ecosystem, community level, belowground

摘要：
氮沉降对生态系统功能和过程具有显著影响。已有研究表明氮添加会影响植物叶片C:N:P化学计量特征。然而,氮添加对植物根系C:N:P化学计量特征影响的研究罕有报道。本论文的主要目的是对比氮添加对草原植物群落地上和地下C:N:P化学计量特征的影响。我们在内蒙古锡林郭勒草原开展了一个7年的氮添加实验,该实验包括6个处理,分别是对照:control; N1: 0.4 mol·m^-2 N; N2: 0.8 mol·m^-2 N; N3: 1.6 mol·m^-2 N; N4: 2.8 mol·m^-2 N; N5: 4 mol·m^-2 N,每个处理6次重复。我们测量并分析了植物地上、地下生物量和C:N:P化学计量特征。研究结果表明,氮添加会导致植物群落地上部分碳浓度降低,氮浓度和磷浓度升高,C:N和C:P比值降低,N:P升高,而且地上碳、氮、磷库均因施氮而增加。然而,氮添加对0-10,10-30,30-50和50-100 cm 4个土层的地下部分碳、氮、磷浓度、比例、库和化学计量特征没有显著影响。综上所述,氮添加会显著改变植物群落地上部分C:N:P化学计量特征,进而影响草地生态系统的功能和过程,但对地下C:N:P化学计量特征几乎没有影响。

关键词: 氮沉降, C:N:P 化学计量, 草地生态系统, 群落, 地下

Ziqi Wang, Jie Wang, Honghui Wu, Tian Yang, Yixin An, Yunlong Zhang, Jianlin Bian, Ying Li, Haiyan Ren, Ariuntsetseg Lkhagva, Xu Wang, Qiang Yu. Nitrogen addition alters aboveground C:N:P stoichiometry of plants but not for belowground in an Inner Mongolia grassland[J]. J Plant Ecol, 2024, 17(1): 0-rtad041.

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Nitrogen addition alters aboveground C:N:P stoichiometry of plants but not for belowground in an Inner Mongolia grassland

氮添加对内蒙古草原植物地上和地下碳氮磷化学计量学特征的影响

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