J Plant Ecol ›› 2023, Vol. 16 ›› Issue (3): rtac091.DOI: 10.1093/jpe/rtac091

• Short Communication •    

Effects of long-term phosphorus addition on soil ratios of phosphomonoesterase to phosphodiesterase in three tropical forests

Taiki Mori1,2,†, Senhao Wang1,3,†, Cong Wang4, Ji Chen5,6,7, Cheng Peng1,3,8, Mianhai Zheng1,3, Juan Huang1,3, Faming Wang1,3, Zhanfeng Liu1,3, Jiangming Mo1,3, Wei Zhang1,3,*   

  1. 1Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China;
    2Kyushu Research Center, Forestry and Forest Products Research Institute, FFPRI, Kurokami 4-11-16, Kumamoto 860-0862, Japan;
    3Center for Plant Ecology, Core Botanical Gardens, Chinese Academy of Sciences, Guangzhou 510650, China;
    4State Key laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;
    5Department of Agroecology, Aarhus University, 8830 Tjele, Denmark;
    6Aarhus University Centre for Circular Bioeconomy, Aarhus University, 8830 Tjele, Denmark;
    7iCLIMATE Interdisciplinary Centre for Climate Change, Aarhus University, 4000 Roskilde, Denmark;
    8University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2022-02-11 Revised:2022-04-17 Accepted:2022-09-17 Online:2022-09-30 Published:2023-06-01
  • Contact: E-mail: zhangwei@scbg.ac.cn
  • About author:† These authors contributed equally to this work.


Abstract: Soil microorganisms in tropical forests can adapt to phosphorus (P)-poor conditions by changing the activity ratios of different types of phosphatases. We tested whether microorganisms in P-poor tropical forest soils increased the phosphomonoesterase (PME) to phosphodiesterase (PDE) activity ratio, because a one-step enzymatic reaction of monoester P degradation might be more adaptive for microbial P acquisition than a two-step reaction of diester P degradation. A continuous 10-year P addition experiment was performed in three tropical forests. The activities of PME and PDE, and their ratio in soil, were determined under the hypothesis that the P-fertilized plots where P shortage is relieved would have lower PME:PDE ratios than the unfertilized controls. We demonstrated that long-term P addition in tropical forest soil did not alter the PME:PDE ratio in primary and secondary forests, whereas P fertilization elevated the PME:PDE ratio in planted forest. These results were in contrast to previous results. The long-term, large-scale P fertilization in our study may have reduced litter- and/or throughfall-derived PDE, which negated the lowered PME:PDE ratio via exogenous P inputs.

Key words: soil extracellular enzymes, phosphomonoestarase, phosphodiesterase, phosphorus fertilization, tropical forest


关键词: 土壤胞外酶, 磷酸单酯酶, 磷酸二酯酶, 施磷, 热带森林