罗浮栲林不同土层微生物碳和氮利用效率对氮添加呈现一致性响应

doi:10.1093/jpe/rtaf183

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罗浮栲林不同土层微生物碳和氮利用效率对氮添加呈现一致性响应

收稿日期:2025-08-22 接受日期:2025-10-30

Parallel patterns of microbial carbon and nitrogen use efficiency in response to nitrogen addition across soil depths in a Castanopsis faberi forest

Yaping Lin^1,2,3, Qiufang Zhang^1,2,3*, Hao Sun^1,2,3, Jiaohong Lu^1,2,3, Zehong Yao^1,2,3, Quanxin Zeng^1,4, Chenxing Lian^1,2,3, Huizhi Pi^1,2,3, Xiaochun Yuan⁵, Yuehmin Chen^1,2,3*

¹Key Laboratory of Humid subtropical Eco-geographical Process of Ministry of Education, School of Geographical Sciences, Fujian Normal University, Fuzhou 350117, China

²Fujian Sanming Forest Ecosystem National Observation and Research Station, Fujian Normal University, Fuzhou 350117, China

³Fujian Provincial Key Laboratory for Subtropical Resources and Environment, Fujian Normal University, Fuzhou 350117, China

⁴State Key Laboratory of Vegetation Structure, Function and Construction (VegLab), Ministry of Education Key Laboratory of Earth Surface Processes, and College of Urban and Environmental Sciences, Peking University, Beijing 100871, China

⁵College of Tourism, Wuyi University, Wuyishan 354300, China.

^*Corresponding author: Qiufang Zhang and Yuehmin Chen
Email: qiufangzh@fjnu.edu.cn (Qiufang Zhang); ymchen@fjnu.edu.cn (Yuehmin Chen)

Received:2025-08-22 Accepted:2025-10-30
Supported by:
This work was supported by the National Natural Science Foundation of China (32471684 and 32371846), the Natural Science Foundation of Fujian Province (2024J01466 and 2023R1002002)

摘要/Abstract

摘要： 微生物碳利用效率(CUE)和氮利用效率(NUE)是决定土壤碳(C)和氮(N)归宿的关键参数。然而，不同土层中微生物CUE和NUE对氮沉降的响应尚不明确。本研究以罗浮栲(Castanopsis faberi)林为对象，通过氮添加试验，采集表层(0–10 cm)和亚表层(20–30 cm)土壤样品，探讨不同土层微生物CUE和NUE对氮添加的响应，并测定土壤碳组分、微生物生物量、酶活性及微生物群落组成等指标。结果显示，微生物CUE与NUE呈显著正相关，揭示了碳氮代谢的耦合关系。氮添加降低了表层和亚表层土壤的CUE和NUE，但在亚表层土壤中的负效应较弱。氮添加显著改变了土壤不同碳组分的积累：在表层土壤中，氮添加增加了易降解碳和颗粒态有机碳含量，而在亚表层土壤中，氮添加减少了矿物结合态有机碳和难降解碳含量。微生物CUE和NUE与土壤碳组分含量显著相关。与表层相比，亚表层土壤的CUE、NUE和可溶性有机碳含量较低，但C/N比更高，表明不同土层CUE和NUE的差异同时受碳数量和质量的调控。本研究揭示了氮添加对微生物资源利用效率的一致负效应及其对土层的依赖性，为深入理解森林生态系统碳氮耦合过程提供了新见解。

关键词: 氮沉降, 微生物生长, 微生物矿化, 土壤深度, 碳组分

Abstract: Microbial carbon use efficiency (CUE) and nitrogen use efficiency (NUE) are key parameters that determine the fate of carbon (C) and nitrogen (N) in soils. However, the responses of microbial CUE and NUE to N deposition across soil depths remain poorly understood. In this study, we conducted an N-addition experiment in a Castanopsis faberi forest. Soil samples were collected from the topsoil (0–10 cm) and subsoil (20–30 cm) under three N addition levels to investigate depth-specific responses of microbial CUE and NUE. Changes in soil C fractions, microbial biomass, enzyme activities, and microbial community composition were assessed. Results showed a significant positive correlation between CUE and NUE, indicating a closed relationship between microbial C and N metabolism. Nitrogen addition reduced CUE and NUE at both depths, though the negative effects were less pronounced in the subsoil. Nitrogen addition significantly altered soil C fractions: labile C and particulate organic C increased in the topsoil, whereas mineral-associated organic C and recalcitrant C decreased in the subsoil. Microbial CUE and NUE were significantly correlated with the quantity of soil C fractions. Compared to the topsoil, the subsoil had lower CUE, NUE, and dissolved organic C content but a higher C/N ratio, suggesting that differences in CUE and NUE between soil depths are regulated by both the quantity and quality of soil C. These findings reveal a depth-dependent yet directionally consistent effect of N addition on microbial resource-use efficiency, offering new insights into C-N coupling processes in forest ecosystems.

Key words: Nitrogen deposition, Microbial growth, Microbial mineralization, Soil depth, Carbon fractions

. 罗浮栲林不同土层微生物碳和氮利用效率对氮添加呈现一致性响应[J]. Journal of Plant Ecology, DOI: 10.1093/jpe/rtaf183.

Yaping Lin, Qiufang Zhang, Hao Sun, Jiaohong Lu, Zehong Yao, Quanxin Zeng, Chenxing Lian, Huizhi Pi, Xiaochun Yuan, Yuehmin Chen. Parallel patterns of microbial carbon and nitrogen use efficiency in response to nitrogen addition across soil depths in a Castanopsis faberi forest[J]. J Plant Ecol, DOI: 10.1093/jpe/rtaf183.

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