• •
Senhao Wang 1,2,3,4#, Kaijun Zhou 5,#, Taiki Mori 1,2,3,6, Andi Li 1,2,3, Cheng Peng 1,2,3,7, Guangcan Yu 1,2,3, Enqing Hou 1, 2, 3, Sihan Yao8, Linjie Fan 1,2,3,9, Baixin Zhang 1,2,3,9, Yufang Wang 1,2,3,10, Qing Ye 1,2,3, Weiren Wang 1,2,3,9, Shun Zou11, Xiangping Tan 1,2,3, Qinggong Mao 1,2,3, Mianhai Zheng 1,2,3, Juan Huang 1,2,3, Zhanfeng Liu1,2,3, Jiangming Mo 1, 2, 3, Wei Zhang 1, 2, 3 *
1Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China 2South China National Garden, Guangzhou 510650, China
3Key laboratory of National Forestry and Grassland Administration on Plant Conservation and Utilization in Southern China, Guangzhou 510650, China
4Guangzhou Academy of Agricultural and Rural Sciences, Guangzhou 510300, China
5The protection center of agricultural environment and cultivated land quality of Guangdong province, Guangzhou 510000, China
6Kyushu Research Center, Forestry and Forest Products Research, Kumamoto, Japan
7Department of Soil Ecology, UFZ-Helmholtz Centre for Environmental Research, Halle (Saale), 06120, Germany
8The Affiliated High School of South China Normal University, Guangzhou 510630, China
9College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 100049, China
10Shiyan Forestry Science Research Institute, Shiyan 442700, China
11Guizhou University of Engineering Science, Bijie 551700, China
*Corresponding author, Zhangwei@scbg.ac.cn
#These authors contributed equally to this work.
摘要： 豆科树种比非豆科树种具备更强的固氮能力，这一特性使其能为磷酸酶合成提供更多氮源，从而具有“磷获取优势”。然而，这种优势能否在长期氮沉降下持续，目前尚不清楚。基于此，本研究在开展长期模拟氮沉降实验研究的第7年，分别采集了豆科与非豆科树种主导的人工林表层土壤，测定其酸性磷酸酶活性。同 时，为评估持续氮添加的效应，本研究还整合了实验第2、8、12、13年的磷酸酶活 性数据。研究发现，长期氮输入后，两种人工林土壤酸性磷酸酶活性的差异逐渐消失，且高氮添加会加速这一过程。其原因在于非豆科树种的磷限制加剧促使其合成更多磷酸酶，而豆科树种土壤氮含量对磷酸酶活性的贡献效率与非豆科相比持续降低，表明土壤氮含量不再主导酸性磷酸酶的活性变化。本研究证实，长期高氮沉降会加速豆科人工林“磷获取优势”的消失。为阐明全球变化下豆科与非豆科树种在 磷利用策略上的差异，未来仍需开展更为系统的研究。
