Changes in soil microbial biomass and community structure with addition of contrasting types of plant litter in a semiarid grassland ecosystem

doi:10.1093/jpe/rtq001

Abstract

Abstract: Aims Elevated atmospheric CO₂ has the potential to enhance the net primary productivity of terrestrial ecosystems. However, the role of soil microorganisms on soil C cycling following this increased available C remains ambiguous. This study was conducted to determine how quality and quantity of plant litter inputs would affect soil microorganisms and consequently C turnover.
Methods Soil microbial biomass and community structure, bacterial community-level physiological profile, and CO₂ emission caused by different substrate C decomposition were investigated using techniques of biological measurements, chemical and stable C isotope analysis, and BIOLOG-ECO microplates in a semiarid grassland ecosystem of northern China in 2006 and 2007 by mixing three contrasting types of plant materials, C₃ shoot litter (SC 3), C₃ root litter (RC 3), and C₄ shoot litter (SC 4), into the 10- to 20-cm soil layer at rates equivalent to 0 (C 0), 60 (C 60), 120 (C 120) and 240 g C m ?2 (C 240).
Important findings Litter addition significantly enriched soil microbial biomass C and N and resulted in changes in microbial structure. Principal component analysis of microbial structure clearly differentiated among zero addition, C₃ -plant-derived litter, and C₄ -plant-derived litter and among shoot- and root-derived litter of C₃ plants; soil microorganisms mainly utilized carbohydrates without litter addition, carboxylic acids with C₃ -plant-derived litter addition and amino acids with C₄ -plant-derived litter addition. We also detected stimulated decomposition of older substrate with C₄ -plant-derived litter inputs. Our results show that both quality and quantity of belowground litter are involved in affecting soil microbial community structure in semiarid grassland ecosystem.

Key words: belowground process, decomposition, plant litter, microbial community, priming effect, semiarid grassland

摘要：
Aims Elevated atmospheric CO₂ has the potential to enhance the net primary productivity of terrestrial ecosystems. However, the role of soil microorganisms on soil C cycling following this increased available C remains ambiguous. This study was conducted to determine how quality and quantity of plant litter inputs would affect soil microorganisms and consequently C turnover.
Methods Soil microbial biomass and community structure, bacterial community-level physiological profile, and CO₂ emission caused by different substrate C decomposition were investigated using techniques of biological measurements, chemical and stable C isotope analysis, and BIOLOG-ECO microplates in a semiarid grassland ecosystem of northern China in 2006 and 2007 by mixing three contrasting types of plant materials, C₃ shoot litter (SC 3), C₃ root litter (RC 3), and C₄ shoot litter (SC 4), into the 10- to 20-cm soil layer at rates equivalent to 0 (C 0), 60 (C 60), 120 (C 120) and 240 g C m ?2 (C 240).
Important findings Litter addition significantly enriched soil microbial biomass C and N and resulted in changes in microbial structure. Principal component analysis of microbial structure clearly differentiated among zero addition, C₃ -plant-derived litter, and C₄ -plant-derived litter and among shoot- and root-derived litter of C₃ plants; soil microorganisms mainly utilized carbohydrates without litter addition, carboxylic acids with C₃ -plant-derived litter addition and amino acids with C₄ -plant-derived litter addition. We also detected stimulated decomposition of older substrate with C₄ -plant-derived litter inputs. Our results show that both quality and quantity of belowground litter are involved in affecting soil microbial community structure in semiarid grassland ecosystem.

Hongmei Jin, Osbert Jianxin Sun, Jianfeng Liu. Changes in soil microbial biomass and community structure with addition of contrasting types of plant litter in a semiarid grassland ecosystem[J]. J Plant Ecol, 2010, 3(3): 209-217.

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[2]	Zuliang Lei, Yexin Ding, Weifeng Xu, Yingjiao Zhang. Microbial community structure in rice rhizosheaths under drought stress [J]. J Plant Ecol, 2023, 16(5): 0-rtad012.
[3]	Xiongde Dong, Leyun Yang, Laura Sofie Harbo, Xinyu Yan, Ji Chen, Cancan Zhao, Yutong Xiao, Hao Liu, Shilin Wang, Yuan Miao, Dong Wang and Shijie Han. Effects of land use on soil microbial community structure and diversity in the Yellow River floodplain [J]. J Plant Ecol, 2023, 16(1): 0-.
[4]	Youli Yu, Huiyuan Cheng, Congyan Wang, and Daolin Du. Heavy drought reduces the decomposition rate of the mixed litters of two composite invasive alien plants [J]. J Plant Ecol, 2023, 16(1): 0-.
[5]	Yang Zhou, Wang-Wang Lv, Shi-Ping Wang, Li-Rong Zhang, Jian-Ping Sun, Li-Li Jiang, Pei-Pei Liu, Qi Wang, Bo-Wen Li, A. Wang, Huan Hong, Su-Ren Zhang, Lu Xia, Nan Ji, Zheng-Xin Xie, Cai-Yun Luo, Zhen-Hua Zhang, Chang-Hui Wang, Jin-Zhi Wang, Ci Yang and Tsechoe Dorji. Additive effects of warming and grazing on fine-root decomposition and loss of nutrients in an alpine meadow [J]. J Plant Ecol, 2022, 15(6): 1273-1284.
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