J Plant Ecol ›› 2022, Vol. 15 ›› Issue (4): 756-770 .DOI: 10.1093/jpe/rtab115

• Research Articles • Previous Articles     Next Articles

Effects of nitrogen addition on clonal integration between mother and daughter ramets of Moso bamboo: a 13C-CO2 pulse labeling study

Wanlu Zhai1, Yi Wang1, Junwei Luan1,*, Shirong Liu2,*   

  1. 1 Key Laboratory of Bamboo and Rattan Science and Technology, State Forestry Administration, Institute of Resources and Environment, International Centre for Bamboo and Rattan, Beijing 100102, China, 2 Key Laboratory of Forest Ecology and Environment of National Forestry and Grassland Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China.

    *Corresponding author. E-mail: liusr@caf.ac.cn (S.L.); junweiluan@icbr.ac.cn (J.L.)
  • Received:2021-04-14 Revised:2021-06-07 Accepted:2021-08-29 Online:2021-10-10 Published:2022-08-01


Resource sharing among connected ramets (i.e. clonal integration) is one of the distinct traits of clonal plants. Clonal integration confers Moso bamboo (Phyllostachys pubescens) a strong adaptability to different environmental conditions. But the mechanisms of how clonal integration makes Moso bamboo has better performance are still poorly understood. In this study, acropetal and basipetal translocation of photosynthates between Moso bamboo ramets were analyzed separately to investigate how clonal fragments obtain higher benefits under heterogeneous N conditions. Clonal fragments of Moso bamboo consisting of two interconnected mother–daughter ramets were used, each of the ramets was subjected to either with or without N addition. The acropetal and basipetal translocation of 13C-photosynthates was separated via single-ramet 13CO2-labeling. Mother ramets translocated more 13C-photosynthates to daughter ramets with N addition, and the translocation of 13C-photosynthates to mother ramets was more pronounced when daughter ramets were treated with N addition. The 13C-photosynthates that were translocated from mother ramets without and with N addition were mainly invested in the leaves and roots of daughter ramets with N addition, from daughter ramets with N addition were mainly invested in the leaves and roots of mother ramets with and without N addition, respectively. These results suggest that mother ramets preferentially invest more resources in nutrient-rich daughter ramets, and that daughter ramets serve as efficient resource acquisition sites to specialize in acquiring abundant resources based on the resource conditions of mother ramets. Clonal plants can improve their resource acquisition efficiency and maximize the overall performance in this way.

Key words: carbon allocation, habitat heterogeneity, 13C-CO2 labeling, resource integration, Moso bamboo

相连分株间的资源共享(即克隆整合)是克隆植物的显著特征。克隆整合使毛竹(Phyllostachys pubescens)对多种环境条件具有较强的适应性。但毛竹通过克隆整合获得性能提升的机制尚不明确。本 研究区分并分析了毛竹光合碳的顶向整合和基向整合,以探究毛竹克隆片段如何在土壤氮异质性条件下 提高整体收益。本研究以由两株不同年龄毛竹分株组成 的克隆片段为研究对象,分株间通过根状茎连接。 每个分株设置氮添加或无氮添加两种处理,并通过单株母株或子株的13C-CO2脉冲标记对光合碳的顶向整合和基向整合进行区分。研究结果表明,子株氮添加显著促进了光合碳由母株向子株的顶向整合,无论母株是否有氮添加,均向氮添加子株转运更多的光合碳。转运自无氮添加母株的光合碳主要分配至氮添加子株的 叶片,而转运自氮添加母株的光合碳则主要分配至根系。与无氮添加处理的子株相比,氮 添 加子株转运更多的光合碳至母株,且该过程不受母株氮添加的影响。转运自氮添加子株的光合碳主要 分配至无氮添加母株的根系和氮添加母株的叶片。这些结果说明,母株优先将更多的资源投入到具有高 养分可利用性的子株,随后子株作为更高效的资源采集点,根据母株的养分状况针对富集资源进行获取。通过这种方式,克隆植物可以降低资源获取成本并提高资源获取效率,最大限度地提升其整体表现。

关键词: 碳分配, 生境异, 质性, 13C-CO2标记, 资源整合, 毛竹