根系分泌物增加微生物生物量，但降低多样性和丰富度：整合分析

doi:10.1093/jpe/rtaf187

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根系分泌物增加微生物生物量，但降低多样性和丰富度：整合分析

收稿日期:2025-06-25 接受日期:2025-10-27

Root exudates increase microbial biomass but decrease diversity and richness: a meta-analysis

Xiao-Chong Zhang¹, Si-Tong Zhang¹, Yolima Carrillo², Hui-Liang Zhai³, Yi-Zhu Zeng³, Min Liu¹, Xiao-Feng Dong¹, Wei Sun^3*, Jian-Ying Ma^1*

¹Key Laboratory of Geographical Processes and Ecological Security in Changbai Mountains, Ministry of Education, School of Geographical Sciences, Northeast Normal University, Changchun, 130024, 7 China.

²Hawkesbury Institute for the Environment, Western Sydney University, Penrith 2573, Australia.

³Institute of Grassland Science, Key Laboratory of Vegetation Ecology of the Ministry of Education, Jilin Songnen Grassland Ecosystem National Observation and Research Station, Northeast Normal University, Changchun 130024, China.

^*To whom correspondence should be addressed: School of Geographical Sciences, Northeast Normal University, 5268 Renmin Street, Changchun City, China. E-mail: sunwei@nenu.edu.cn (W. Sun); majy652@nenu.edu.cn (J. Ma).

Received:2025-06-25 Accepted:2025-10-27
Supported by:
This research work was supported by the Natural Science Foundation of Jilin Province (20230101171JC), National Natural Science Foundation of China (31870456), the Program of Introducing Talents of Discipline to Universities (B16011).

摘要/Abstract

摘要： 虽然植物根系分泌物在调节土壤微生物群落方面发挥着至关重要的作用，但由于其成分复杂、浓度低及易被降解使得很难确定其对微生物生物量、多样性和结构的影响。在本研究中，我们整合了121篇文献中的3174个观察数据，系统地探究根系分泌物添加对土壤微生物群落的影响及其响应机制。结果表明，分泌物添加显著提高微生物生物量(总微生物生物量：+18.65%；细菌生物量：+31.35%；真菌生物量：+37.73%)，但降低α多样性(-6.17%)、丰富度(-9.16%)和均匀度(-10.09%)，同时还显著影响群落结构(+212.65%)。天然分泌物因其组成复杂和养分含量高，对微生物生物量的促进作用更强，而合成分泌物通过导致某些细菌类群的过度生长，抑制了微生物群落的多样性。土壤有效氮含量被认定为微生物生物量的关键预测指标，而土壤pH则是微生物丰富度和均匀度的重要预测因素。同时，根系分泌物碳输入速率显著抑制了α多样性，并改变了微生物群落结构。此外，分泌物的碳氮比在调节β多样性及群落结构方面起着关键作用。本研究强调了分泌物化学性质、实验条件及土壤物理化学特性的重要性，为研究根分泌物对微生物群落的调控机制提供了新视角。

关键词: 根系分泌物, 微生物生物量, 多样性, 丰富度, 微生物结构, 整合分析

Abstract: While plant root exudates play a crucial role in regulating soil microbial communities, their complex composition, low concentration and degradability make it difficult to determine their effects on microbial biomass, diversity and structure. In this study, we integrated 3,174 observations from 121 publications to systematically assess the effects of root exudate addition on soil microbial communities and their differential response mechanisms. The results indicated that exudates significantly increased microbial biomass (total microbial biomass: + 18.65%; bacteria biomass: + 31.35%; fungi biomass: + 37.73%) but decreased α-diversity (− 6.17%), richness (− 9.16%) and evenness (− 10.09%), while also altered the community structure. Natural exudates, with their complex composition and higher nutrient content, had a stronger effect on promoting microbial biomass, whereas synthetic exudates led to the overgrowth of certain bacterial taxa, suppressing microbial community diversity. The responses of soil available nitrogen content were identified as key predictors of microbial biomass, while soil pH was an important predictor of microbial richness and evenness. Meanwhile, the carbon input rate from root exudates significantly suppressed α-diversity and altered microbial community structure. Additionally, the C:N ratio of exudates played a critical role in regulating β-diversity and community structure. This study highlights the importance of exudate chemical properties, experimental conditions and soil physicochemical characteristics, offering a new perspective on the mechanisms regulating microbial communities by root exudates.

Key words: root exudates, microbial biomass, diversity, richness, microbial structure, meta-analysis

. 根系分泌物增加微生物生物量，但降低多样性和丰富度：整合分析[J]. Journal of Plant Ecology, DOI: 10.1093/jpe/rtaf187.

Xiao-Chong Zhang, Si-Tong Zhang, Yolima Carrillo, Hui-Liang Zhai, Yi-Zhu Zeng, Min Liu, Xiao-Feng Dong, Wei Sun, Jian-Ying Ma. Root exudates increase microbial biomass but decrease diversity and richness: a meta-analysis[J]. J Plant Ecol, DOI: 10.1093/jpe/rtaf187.

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