Journal of Plant Ecology ›› 2023, Vol. 16 ›› Issue (3): 0-rtac091.DOI: 10.1093/jpe/rtac091

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  • 收稿日期:2022-02-11 修回日期:2022-04-17 接受日期:2022-09-17 出版日期:2023-06-01 发布日期:2022-09-30

Effects of long-term phosphorus addition on soil ratios of phosphomonoesterase to phosphodiesterase in three tropical forests

Taiki Mori1,2,†, Senhao Wang1,3,†, Cong Wang4, Ji Chen5,6,7, Cheng Peng1,3,8, Mianhai Zheng1,3, Juan Huang1,3, Faming Wang1,3, Zhanfeng Liu1,3, Jiangming Mo1,3, Wei Zhang1,3,*   

  1. 1Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China;
    2Kyushu Research Center, Forestry and Forest Products Research Institute, FFPRI, Kurokami 4-11-16, Kumamoto 860-0862, Japan;
    3Center for Plant Ecology, Core Botanical Gardens, Chinese Academy of Sciences, Guangzhou 510650, China;
    4State Key laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;
    5Department of Agroecology, Aarhus University, 8830 Tjele, Denmark;
    6Aarhus University Centre for Circular Bioeconomy, Aarhus University, 8830 Tjele, Denmark;
    7iCLIMATE Interdisciplinary Centre for Climate Change, Aarhus University, 4000 Roskilde, Denmark;
    8University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2022-02-11 Revised:2022-04-17 Accepted:2022-09-17 Online:2023-06-01 Published:2022-09-30
  • Contact: E-mail:
  • About author:† These authors contributed equally to this work.

摘要: 热带森林土壤微生物可以通过改变不同类型的磷酸酶活性来适应低磷的环境。以往的研究认为,低磷环境中土壤微生物更倾向于分解有机磷单酯键而非二酯键。因此,磷酸单酯酶(PME)的活性变化对生态系统磷输入的响应可能更敏感,其与磷酸二酯酶(PDE)活性的比值在磷输入后将显著降低。为了检验土壤微生物在低磷的热带森林中对不同磷酸酶的“投资”策略,我们在鼎湖山3种热带森林进行了长达10年的磷添加实验。与以往的研究不同,本研究发现长期磷添加没有改变原生林与次生林的PME:PDE比值,但显著提高了人工林土壤PME:PDE比值。结果表明,长期磷添加可能减少了凋落物或穿透雨来源的PDE,从而使其土壤PDE活性下降较PME降低更快,因而PME:PDE比值未能降低。

关键词: 土壤胞外酶, 磷酸单酯酶, 磷酸二酯酶, 施磷, 热带森林

Abstract: Soil microorganisms in tropical forests can adapt to phosphorus (P)-poor conditions by changing the activity ratios of different types of phosphatases. We tested whether microorganisms in P-poor tropical forest soils increased the phosphomonoesterase (PME) to phosphodiesterase (PDE) activity ratio, because a one-step enzymatic reaction of monoester P degradation might be more adaptive for microbial P acquisition than a two-step reaction of diester P degradation. A continuous 10-year P addition experiment was performed in three tropical forests. The activities of PME and PDE, and their ratio in soil, were determined under the hypothesis that the P-fertilized plots where P shortage is relieved would have lower PME:PDE ratios than the unfertilized controls. We demonstrated that long-term P addition in tropical forest soil did not alter the PME:PDE ratio in primary and secondary forests, whereas P fertilization elevated the PME:PDE ratio in planted forest. These results were in contrast to previous results. The long-term, large-scale P fertilization in our study may have reduced litter- and/or throughfall-derived PDE, which negated the lowered PME:PDE ratio via exogenous P inputs.

Key words: soil extracellular enzymes, phosphomonoestarase, phosphodiesterase, phosphorus fertilization, tropical forest