接种丛枝菌根真菌和养分添加对芦苇功能性状的联合影响

doi:10.1093/jpe/rtaf169

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接种丛枝菌根真菌和养分添加对芦苇功能性状的联合影响

收稿日期:2025-07-25 接受日期:2025-09-23

Combined effects of arbuscular mycorrhizal fungi and nutrient addition on the functional traits of Phragmites australis

Rong Hu ^a, Mengxuan Wang ^b, Aixin Hou ^c, Xiaohong Guo ^b, Xiang Long ^a, Ying Xu ^d, Feilong Huang ^b, Sifan Peng ^a, Fanzhu Qu ^a, Xuehong Wang ^a, Zhikang Wang ^a, Yating Li ^b, Bo Guan ^a,*

a The Institute for Advanced Study of Coastal Ecology, Ludong University, Yantai 264025, China

b School of Resources and Environmental Engineering, Ludong University, Yantai 264025, China

c Department of Environmental Sciences, College of the Coast and Environment, Louisiana State University, Baton Rouge, LA, 70803, USA

d School of Environment, Northeast Normal University, Changchun, 130117, China

*Corresponding author: Bo Guan, Email: bguan@ldu.edu.cn

Received:2025-07-25 Accepted:2025-09-23
Supported by:
This work was financially supported by the National Natural Science Foundation of China (42471111 and 32171649), the Natural Science Foundation of Shandong Province (ZR2023MD076), Youth Innovation Teams in Colleges and Universities of Shandong Province (2022KJ118), the Key R&D Program of Shandong Province, China (2024KJHZ003) and the Innovation Project for graduate students of Ludong University (810201)

摘要/Abstract

摘要： 丛枝菌根真菌（AMF）通过促进养分吸收能够显著增强陆生植物的生长。然而，目前对于AMF与养分输入的交互作用对植物功能性状及其权衡关系的影响仍缺乏深入认识。本研究以芦苇（Phragmites australis）为宿主植物，利用盆栽实验设置了AMF接种与未接种处理，并施加了三种氮添加水平和两种磷添加水平。结果表明，接种AMF显著提高了芦苇根系的AMF侵染率。在未施肥条件下，接种AMF显著促进了植物形态生长与生物量积累。氮添加主要促进了芦苇地上部分的生长，而磷添加则显著促进了根系的发育。在光合性状方面，接种AMF显著提高了叶片类胡萝卜素含量，而磷添加则使其净光合速率、蒸腾速率和气孔导度分别显著降低了18.5%、25.8%和28.2%。此外，接种AMF提高了叶片氮、磷含量，但降低了茎氮含量以及须根、根状茎和茎的N:P比。性状网络分析结果显示，接种AMF使网络的中心由叶生物量转移向根状茎生物量，反映了枢纽性状从地上部分向地下部分的转变。综上所述，接种AMF显著促进了芦苇生长，但其效应受土壤氮磷供应状况的显著调控，在养分相对贫瘠的条件下菌根效益更为显著。本研究加深了对湿地生态系统中养分—AMF—植物互作关系的理解，并为脆弱水域生境的生态恢复与养分管理提供了理论依据。

关键词: 丛枝菌根真菌, 氮输入, 磷输入, 功能形状, 性状网络

Abstract: Arbuscular mycorrhizal fungi (AMF) can enhance terrestrial plant growth by promoting nutrient uptake. However, the effects of interactions between AMF and nutrient inputs on plant functional traits and their trade-offs remain poorly understood. In this study, a pot experiment was conducted using Phragmites australis as the host species, with AMF inoculation and non-inoculation treatments under three levels of nitrogen addition and two levels of phosphorus addition. The results showed that AMF inoculation significantly increased AMF colonization in the roots of P. australis. Under unfertilized conditions, AMF significantly promoted plant morphological development and biomass accumulation. Nitrogen addition primarily enhanced aboveground growth, while phosphorus addition significantly stimulated root system development. Regarding photosynthetic traits, AMF inoculation significantly increased carotenoid content, whereas phosphorus addition significantly reduced net photosynthetic rate (Pn), transpiration rate (Tr), and stomatal conductance (Gs) by 18.5%, 25.8%, and 28.2%, respectively. Furthermore, AMF inoculation increased leaf nitrogen and phosphorus contents but reduced stem nitrogen content and the N:P ratios in fine roots, rhizomes, and stems. Trait network analysis revealed that AMF inoculation shifted the central traits from leaf biomass to rhizome biomass, indicating a transition in hub traits from aboveground to belowground organs. These findings suggest that AMF inoculation significantly promotes the growth of P. australis although the magnitude of this benefit is modulated by soil nitrogen and phosphorus availability, with stronger effects observed under nutrient-poor conditions. This study advances our understanding of nutrient–AMF–plant interactions in wetland ecosystems and provides a theoretical foundation for ecological restoration and nutrient management in vulnerable aquatic habitats.

Key words: Arbuscular mycorrhizal fungi, N input, P input, functional traits, trait networks

. 接种丛枝菌根真菌和养分添加对芦苇功能性状的联合影响[J]. Journal of Plant Ecology, DOI: 10.1093/jpe/rtaf169.

Rong Hu, Mengxuan Wang, Aixin Hou, Xiaohong Guo, Xiang Long, Ying Xu, Feilong Huang, Sifan Peng, Fanzhu Qu, Xuehong Wang, Zhikang Wang, Yating Li, Bo Guan. Combined effects of arbuscular mycorrhizal fungi and nutrient addition on the functional traits of Phragmites australis[J]. J Plant Ecol, DOI: 10.1093/jpe/rtaf169.

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[2]	. 胡杨异形叶片的性状变异及其相关网络的对比研究[J]. Journal of Plant Ecology, 2025, 18(2): 1-13.
[3]	. 低磷环境下宿主植物的丛枝菌根状态影响其后代的表型和转录组表达[J]. Journal of Plant Ecology, 2024, 17(2): 0-rtae014.
[4]	. 复合非生物胁迫下丛枝菌根真菌对玉米碳同化和生态化学计量的影响[J]. Journal of Plant Ecology, 2024, 17(2): 0-rtae010.
[5]	. [J]. Journal of Plant Ecology, 2022, 15(5): 1007-1020.
[6]	. [J]. Journal of Plant Ecology, 2022, 15(3): 581-595.
[7]	. [J]. Journal of Plant Ecology, 2022, 15(3): 437-449.
[8]	. [J]. Journal of Plant Ecology, 2022, 15(2): 399-412.
[9]	. [J]. Journal of Plant Ecology, 2021, 14(5): 945-958.
[10]	. [J]. Journal of Plant Ecology, 2020, 13(4): 478-488.