J Plant Ecol ›› 2016, Vol. 9 ›› Issue (2): 201-211.doi: 10.1093/jpe/rtv037

• Research Articles • Previous Articles     Next Articles

Species richness loss after nutrient addition as affected by N:C ratios and phytohormone GA3 contents in an alpine meadow community

Xiaomei Sun1, Kailiang Yu2, Herman H. Shugart2 and Gang Wang1,*   

  1. 1 State Key Laboratory of Grassland Agro-Ecosystem, School of Life Sciences, Lanzhou University, 222 Tianshui Road, Chengguan District, Lanzhou, Gansu 730000, China; 2 Department of Environmental Sciences, University of Virginia, 291 McCormick Road, Charlottesville, VA 22904, USA
  • Received:2014-10-27 Accepted:2015-04-15 Online:2015-04-20 Published:2016-03-24
  • Contact: Wang, Gang E-mail:wgmg36@lzu.edu.cn

Abstract: Aims From the light-competition hypothesis, competition for light is asymmetric and the observed increases in plant-size variability with increasingly denser canopies are primarily due to competition for light. Greater plant height provides pre-emptive access to light and produces increased height differences among species. The question is what produces these differences in plant height or height growth response among species in response to fertilization.
Methods In 2009, a field experiment of N, P and N + P enrichments at three levels each was initiated in an alpine meadow on the northeast Qinghai-Tibet Plateau. Effects of fertilization on species richness, aboveground net primary production (ANPP), relative light intensity and plant height of different plant functional groups were determined. Festuca ovina (grass), Kobresia humilis (sedge), Oxytropis ochrocephala (legume), Taraxacum lugubre (rosette forb) and Geranium pylzowianum (upright forb) were selected as exemplars of each of the indicated functional groups. The N:C ratios in aboveground biomass, gibberellic acid (GA 3) concentrations in leaves, plant heights and height relative growth rate (RGR) of these exemplar species were analyzed in detail.
Important findings Species richness of grasses significantly increased with increasing N + P levels. Species richness of legumes and upright forbs decreased after N and N + P additions. P addition had no significant effect on species richness. The effects of N + P addition on species richness and ANPP were consistently stronger than those of the single N or P fertilization. Reductions in species richness caused by nutrient addition paralleled the increases in ANPP and decreases in light intensity under the canopies, indicating indirect effect of nutrient addition on species richness via ANPP-induced light competition. The exemplar species that responded most positively to fertilization in height and RGR also displayed stronger increases in their GA 3 content and N:C ratios. GA 3 concentrations and N:C ratios were positively correlated with height RGR when the data were pooled for all species. The tallest and the fastest-growing grass, F. ovina, had the largest increase in N:C ratios and the highest leaf GA 3 concentrations after nutrient addition. These results indicated that differential responses of GA 3 concentrations and N:C ratios to fertilization were related to the inequality in plant heights among species.

Key words: nutrient addition, N:C ratio, GA3 content, asymmetry in plant heights, competition for light, species richness loss

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