J Plant Ecol ›› 2009, Vol. 2 ›› Issue (3): 143-151.doi: 10.1093/jpe/rtp016

• Research Articles • Previous Articles     Next Articles

Assessing genetic diversity and structure of fragmented populations of eastern white pine (Pinus strobus) and western white pine (P. monticola) for conservation management

Melanie Mehes, Kabwe K. Nkongolo* and Paul Michael   

  1. Department of Biology, Laurentian University, Sudbury, Ontario, Canada P3E 2C6
  • Received:2009-05-05 Accepted:2009-07-27 Online:2009-08-13 Published:2009-08-26
  • Contact: Nkongolo, Kabwe E-mail:knkongolo@laurentian.ca

Abstract: Aims Many pine populations in Canada have fragmented distributions resulting from the effects of glaciations, overharvesting and white pine blister rust infections. Forest fragmentation can modify gene flow and reduce genetic diversity. Selective logging can reduce the density of trees, thereby altering mating patterns and increasing inbreeding. The hypothesis of the present study is that forest fragmentation will not increase inbreeding and will have no effect on genetic diversity parameters in the Canadian Pinus moniticola and P. strobus populations targeted because of (i) the long life span of the pine species, (ii) outbreeding and self-incompatibility of P. monticola and P. strobus and (iii) wind pollination resulting in high gene flow among populations. We studied the genetic diversity of P. strobus across its range in Canada, and we completed a detailed analysis of the genetic structure of P. monticola populations from western Canada using microsatellites genetic markers.
Methods Seed samples from 10 P. monticola populations and 10 P. strobus populations were collected from western and eastern Canada, respectively. The mother trees included in seed lots were representative of each stand. Genomic DNA extracted from each sample was amplified with microsatellite primers. The intra- and interpopulation genetic diversity parameters were assessed using Popgene and Genepop softwares and the genetic distances among populations within each species using the PowerMarker software.
Important findings Pinus monticola and P. strobus exhibited moderate to high genetic diversity. Also, both species showed low levels of inbreeding despite the geographic isolation and small stand size. Gene flow estimates were high and population differentiation values were relatively low for these fragmented forest sites.

Key words: Pinus strobus, Pinus monticola, microsatellite, genetic diversity, forest fragmentation

[1] Maria Šurinová, Věroslava Hadincová, Vigdis Vandvik and Zuzana Münzbergová. Temperature and precipitation, but not geographic distance, explain genetic relatedness among populations in the perennial grass Festuca rubra [J]. J Plant Ecol, 2019, 12(4): 730-741.
[2] Xinzeng Wei, Dachuan Bao, Hongjie Meng and Mingxi Jiang. Pattern and drivers of speciesgenetic diversity correlation in natural forest tree communities across a biodiversity hotspot [J]. J Plant Ecol, 2018, 11(5): 761-770.
[3] Kai Jiang, Po-Keung Eric Tsang, Na-Na Xu and Xiao-Yong Chen. High genetic diversity and strong differentiation in dramatically fluctuating populations of Zostera japonica (Zosteraceae): implication for conservation [J]. J Plant Ecol, 2018, 11(5): 789-797.
[4] David U. Nagy, Szilvia Stranczinger, Aliz Godi, Anett Weisz, Christoph Rosche, Jan Suda, Mark Mariano, Robert W. Pal. Does higher ploidy level increase the risk of invasion? A case study with two geo-cytotypes of Solidago gigantea Aiton (Asteraceae) [J]. J Plant Ecol, 2018, 11(2): 317-327.
[5] Amaranta Arellano-Rivas, J. Arturo De-Nova, Miguel A. Munguía-Rosas. Patch isolation and shape predict plant functional diversity in a naturally fragmented forest [J]. J Plant Ecol, 2018, 11(1): 136-146.
[6] Matteo Brezzi, Bernhard Schmid, Pascal A. Niklaus, Andreas Schuldt. Tree diversity increases levels of herbivore damage in a subtropical forest canopy: evidence for dietary mixing by arthropods? [J]. J Plant Ecol, 2017, 10(1): 13-27.
[7] Christoph Z. Hahn, Stefan G. Michalski, Markus Fischer, Walter Durka. Genetic diversity and differentiation follow secondary succession in a multi-species study on woody plants from subtropical China [J]. J Plant Ecol, 2017, 10(1): 213-221.
[8] Christoph Z. Hahn, Pascal A. Niklaus, Helge Bruelheide, Stefan G. Michalski, Miaomiao Shi, Xuefei Yang, Xueqin Zeng, Markus Fischer, Walter Durka. Opposing intra vs. interspecific diversity effects on herbivory and growth in subtropical experimental tree assemblages [J]. J Plant Ecol, 2017, 10(1): 242-251.
[9] Shan Yuan, Chengyuan Guo, Linna Ma, Renzhong Wang. Environmental conditions and genetic differentiation: what drives the divergence of coexisting Leymus chinensis ecotypes in a large-scale longitudinal gradient? [J]. J Plant Ecol, 2016, 9(5): 616-628.
[10] Elke Seeber, Georg Miehe, Isabell Hensen, Yongping Yang,, Karsten Wesche. Mixed reproduction strategy and polyploidy facilitate dominance of Kobresia pygmaea on the Tibetan Plateau [J]. J Plant Ecol, 2016, 9(1): 87-99.
[11] Joaquín Ortego, Raúl Bonal, Alberto Muñoz, José Miguel Aparicio. Extensive pollen immigration and no evidence of disrupted mating patterns or reproduction in a highly fragmented holm oak stand [J]. J Plant Ecol, 2014, 7(4): 384-395.
[12] Zhichao Pu, Poonim Daya, Jiaqi Tan, Lin Jiang. Phylogenetic diversity stabilizes community biomass [J]. J Plant Ecol, 2014, 7(2): 176-187.
[13] Timothy J. S. Whitfeld, Alexandra G. Lodge, Alexander M. Roth, Peter B. Reich. Community phylogenetic diversity and abiotic site characteristics influence abundance of the invasive plant Rhamnus cathartica L. [J]. J Plant Ecol, 2014, 7(2): 202-209.
[14] Nidal Odat, Frank H. Hellwig, Gottfried Jetschke, Markus Fischer. On the relationship between plant species diversity and genetic diversity of Plantago lanceolata (Plantaginaceae) within and between grassland communities [J]. J Plant Ecol, 2010, 3(1): 41-48.
[15] Yann Hautier, Christophe F. Randin, Jürg Stöcklin, Antoine Guisan. Changes in reproductive investment with altitude in an alpine plant [J]. J Plant Ecol, 2009, 2(3): 125-134.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Huang Jian-qin Zhou Jian Fan Ru-wen. The Observation of Cell phytomorphology of the Double Fertilization and Embryogenesis in Liriodendron Chinense( Hemsl) Sary[J]. Chin Bull Bot, 1995, 12(03): 45 -47 .
[2] QUAN Hong SHI He-Ping LI Ling. Signal Transduction of ABA Inducing Stomatal Closure[J]. Chin Bull Bot, 2003, 20(06): 664 -670 .
[3] LIU Jun XIE Cong-Hua. The Mechanism of Potato (Solanum tuberosum L.) Tuber Development and Related Gene Expression[J]. Chin Bull Bot, 2001, 18(05): 531 -539 .
[4] Jia Zhi-wang and Wu Xue-zhou. Four Channel Data Acquisition on Line from LKB-1217 Liquid Scintillation Counter[J]. Chin Bull Bot, 1990, 7(02): 55 -57 .
[5] Yan Liu;Lu Li. Preliminary Analysis on the Similarity Coefficient Study of Representative Jurassic Floras from China[J]. Chin Bull Bot, 2006, 23(4): 380 -388 .
[6] Li Guo-feng;Wu Zheng-rong;Ye He-chun;Lu Fu-sun and Xiang Gui-qiong. Induction and Formation of the Naphthoqunone Pigments in Vitro in Arnebiaenchroma[J]. Chin Bull Bot, 1988, 5(02): 84 -86 .
[7] Ni De-xiang;Zang Pi-fang;Dong Chong-mei;Zhang Ru-ping and Wang Kai-ji. The Effect of Magnetic Field Intensity on the Shoot Growth of Dianthus caryophyllus L. in Vitro[J]. Chin Bull Bot, 1984, 2(06): 30 -31 .
[8] Guan Kang-lin. A Studies on Light Germination in Paulownia and Casuarina[J]. Chin Bull Bot, 1985, 3(02): 19 -20 .
[9] Yao Jing-xia Li Hao-bing Zhong Shao-bin Wu He-ming. Wheat X maige Hybridization and Wheat Haploid Production[J]. Chin Bull Bot, 1995, 12(03): 31 -35 .
[10] Li Xian-zhang. Life Extension of the Cut- flower of Alstromeria aurantiaca with Floral Preservatives[J]. Chin Bull Bot, 1997, 14(04): 55 -56 .