J Plant Ecol ›› 2009, Vol. 2 ›› Issue (3): 107-118.doi: 10.1093/jpe/rtp015

• Review Article •     Next Articles

Use of 15N stable isotope to quantify nitrogen transfer between mycorrhizal plants

Xinhua He1,2,*, Minggang Xu3, Guo Yu Qiu4,5 and Jianbin Zhou6   

  1. 1 School of Life Sciences, Yunnan Normal University, Kunming, Yunnan 650092, China; 2 School of Plant Biology (M084), University of Western Australia, Crawley, Western Australia 6009, Australia; 3 Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 4 Key Laboratory for Environmental and Urban Sciences, Shenzhen Graduate School, Peking University, Shenzhen 518055, China; 5 State Key Laboratory of Earth Surface Processes and Resource Ecology, Beijing Normal University, Beijing 100875, China; 6 College of Resource and Environmental Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China
  • Received:2009-04-20 Accepted:2009-07-28 Online:2009-08-18 Published:2009-08-26
  • Contact: He, Xinhua E-mail:xinhua5658@hotmail.com

Abstract: Aims Mycorrhizas (fungal roots) play vital roles in plant nutrient acquisition, performance and productivity in terrestrial ecosystems. Arbuscular mycorrhizas (AM) and ectomycorrhizas (EM) are mostly important since soil nutrients, including NH4+, NO3? and phosphorus, are translocated from mycorrhizal fungi to plants. Individual species, genera and even families of plants could be interconnected by mycorrhizal mycelia to form common mycorrhizal networks (CMNs). The function of CMNs is to provide pathways for movement or transfer of nutrients from one plant to another. In the past four decades, both 15N external labeling or enrichment (usually expressed as atom%) and 15N naturally occurring abundance (δ15N, ‰) techniques have been employed to trace the direction and magnitude of N transfer between plants, with their own advantages and limitations.

Important Findings

The heavier stable isotope 15N is discriminated against 14N during biochemical, biogeochemical and physiological processes, due to a greater atomic mass. In general, non-N2-fixing plants had greater δ15N values than N2-fixing (~0‰) ones. Foliar δ15N often varied by 5 to 10‰ in the order: non-mycorrhizas/AMs > EMs ≥ ericoid mycorrhizas. Differences in δ15N (‰) or 15N (atom%) values could thus provide N transfer information between plants. A range of between 0 to 80% of one-way N transfer had been observed from N2-fixing mycorrhizal to non-N2-fixing mycorrhizal plants, but generally less than or around 10% in the reverse direction. Plant-to-plant N transfer may provide practical implications for plant performance in N-limited habitats. Considering that N translocation or cycling is crucial, and the potential benefits of N transfer are great in both agricultural and natural ecosystems, more research is warranted on either one-way or two-way N transfers mediated by CMNs with different species and under field conditions.

Key words: 15N enrichment, 15N natural abundance (15N), 15N stable isotope, common mycorrhizal networks (CMNs), nitrogen transfer

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[1] Wei Zhou Hong Wang . The physiological and molecular mechanisms of calcium uptake, transport, and metabolism in plants.[J]. Chin Bull Bot, 2007, 24(06): 762 -778 .
[2] CAO Shuang-He ZHANG Xiang-Qi ZHANG Ai-Min. Review of the Molecular Regulation Mechanism and Genetics of Photoperiod- and/or Thermosensitive Male Sterility[J]. Chin Bull Bot, 2005, 22(01): 19 -26 .
[3] Ma Ke-ping. Studies on the Structure and Function of Calamagrostis angustifolia Grassland Ecosystem I. the Basic Characteristics of Plant Community and Environment[J]. Chin Bull Bot, 1995, 12(专辑2): 1 -8 .
[4] Zhang Zi-duan;Zhao Ji-yong;Men Hu-yen;Wang Yu-ling;Zhou Zhan and Han Rong. Preliminary Report on the Comparative Studies of Karyotypes in Gossypium hirsutum and G. barbadense[J]. Chin Bull Bot, 1988, 5(03): 173 -175 .
[5] Fudou Zhang, Tianlin Li, Gaofeng Xu, Di Wu, Yuhua Zhang. Comparative Analysis of Growth Types and Reproductive Characteristics of Mikania micrantha[J]. Chin Bull Bot, 2011, 46(1): 59 -66 .
[6] Pu Tong-liang and Lu Zhong-shu. The Effects of Endogenous GA and Gas Permeability Through the Hull and Testa on the Dormancy of Arena fatua[J]. Chin Bull Bot, 1988, 5(04): 232 -235 .
[7] Sun De-lan;Zhao Yu-jing and Su Xiu-zhen. Microphotometric Determination of Amyloplasts DNA Content in Cotyledon Cell of Lotus[J]. Chin Bull Bot, 1997, 14(01): 44 -47 .
[8] . [J]. Chin Bull Bot, 1994, 11(专辑): 8 .
[9] Ying Gao, Jianqiang Guo, Jinfeng Zhao. Molecular Mechanisms of Arabidopsis Trichome Development[J]. Chin Bull Bot, 2011, 46(1): 119 -127 .
[10] Shangguan Zhou-ping and Chen Pei-yuan. Responses of Maize Seedings to Osmotic Stress and Physiological Adaptation[J]. Chin Bull Bot, 1990, 7(03): 30 -33 .