杀虫剂施用和植物性别对叶片生理特性和叶际细菌群落的影响

doi:10.1093/jpe/rtac084

Journal of Plant Ecology ›› 2023, Vol. 16 ›› Issue (2): 0-rtac084.DOI: 10.1093/jpe/rtac084

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杀虫剂施用和植物性别对叶片生理特性和叶际细菌群落的影响

收稿日期:2022-04-22 修回日期:2022-06-12 接受日期:2022-08-13 出版日期:2023-04-01 发布日期:2022-08-24

Effects of pesticide application and plant sexual identity on leaf physiological traits and phyllosphere bacterial communities

Zuodong Zhu^1,†, Yue He^2,†, Jiahui Xu¹, Zhenghu Zhou^3,4, Amit Kumar⁵, Zhichao Xia^1,6,*

¹College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China;
²Key Laboratory for Bio- resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China;
³3Center for Ecological Research, Northeast Forestry University, Harbin 150040, China;
⁴Key Laboratory of Sustainable Forest Ecosystem Management—Ministry of Education, Northeast Forestry University, Harbin 150040, China;
⁵Institute of Ecology, Leuphana University of Lüneburg, Lüneburg 21335, Germany;
⁶School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei 230036, China

Received:2022-04-22 Revised:2022-06-12 Accepted:2022-08-13 Online:2023-04-01 Published:2022-08-24
Contact: E-mail: zhichaoxia0623@163.com
About author:^†These authors contributed equally to this work.

摘要/Abstract

摘要： 在全球范围内,杀虫剂被广泛应用于提高粮食产量。然而,关于施用杀虫剂对雌雄异株植物的叶片生理和叶际细菌群落影响的信息很少。因此,本研究旨在评估高效氯氟氰菊酯(一种广谱杀虫剂)对雌雄异株青杨(Populus cathayana)叶片生理和叶际细菌群落的影响。雄性青杨叶片的光合参数(净光合速率(Pn)、气孔导度(gs)和蒸腾作用(E))显著高于雌性,且与杀虫剂的施用无关。但施用杀虫剂后,雌性和雄性的光合相关参数均显著降低。相对于雌性,雄性的降低幅度更大。同时,施用杀虫剂导致雄性叶片的过氧化物酶(POD)活性和丙二醛(MDA)含量显著增加,而超氧化物歧化酶(SOD)活性和总叶绿素含量保持不变。叶际细菌群落表现出一定的保守特性,如辛普森(Simpson)和香农(Shannon)多样性指数不受性别和杀虫剂施用的影响。然而,雌性和雄性青杨的叶际细菌群落的组成存在差异,表明内在的性别特性显著塑造了叶际细菌群落。另一方面,杀虫剂的施用显著增加了放线菌门(Actinobacteria)的相对丰度,但却减少了变形菌门(Proteobacteria)的相对丰度。主成分分析表明,叶片生理与特定细菌类群间存在关联。例如,变形菌门(Proteobacteria)与叶片SOD活性和MDA含量呈负相关,而放线菌门(Actinobacteria)则表现出相反的模式。我们的研究强调雌雄异株植物的叶际细菌群落组成和叶片生理特征存在性别特异性。

关键词: 雌雄异株植物, 高效氯氟氰菊酯, 叶片生理, 叶际细菌群落, 性别特异性响应

Abstract: Pesticides are widely used to enhance food production on a global scale. However, little information is available on the effects of pesticide application on leaf physiology and phyllosphere bacterial communities of dioecious plants. Therefore, this study aimed to assess the impact of λ-cyhalothrin, a broad-spectrum pesticide, on leaf physiology and phyllosphere bacterial communities in the dioecious Populus cathayana. Physiological leaf traits such as photosynthetic apparatus (net photosynthetic rate (Pn), stomatal conductance (gs) and transpiration (E)) of males were significantly higher than those of females, independent of pesticide use. In contrast, pesticide application significantly reduced the photosynthetic apparatus for both sexes, and the reduction was greater in males relative to females. Also, pesticide application significantly increased peroxidase (POD) activity and malondialdehyde (MDA) content and maintained superoxide dismutase (SOD) activity and total chlorophyll content in leaves of males. The phyllosphere bacteria showed some conserved characteristics, in which, Simpson and Shannon diversity indices were not affected by sex or pesticide application. Phyllosphere bacterial community composition differed between females and males, indicating that intrinsic sex significantly shapes the phyllosphere bacterial community. However, pesticide application significantly increased the relative abundance of Actinobacteria but reduced the relative abundance of Proteobacteria. Principal component analysis showed associations between leaf physiology and specific bacterial taxa. For instance, Proteobacteria negatively correlated with leaf SOD activity and MDA content, while Actinobacteria showed an opposite pattern. Our study highlights sex-specific phyllosphere bacterial community composition and leaf physiological traits in dioecious plants.

Key words: dioecious plants, λ-cyhalothrin;, leaf physiology, phyllosphere bacterial community, sex-specific responses

. 杀虫剂施用和植物性别对叶片生理特性和叶际细菌群落的影响[J]. Journal of Plant Ecology, 2023, 16(2): 0-rtac084.

Zuodong Zhu, Yue He, Jiahui Xu, Zhenghu Zhou, Amit Kumar, Zhichao Xia. Effects of pesticide application and plant sexual identity on leaf physiological traits and phyllosphere bacterial communities[J]. J Plant Ecol, 2023, 16(2): 0-rtac084.

杀虫剂施用和植物性别对叶片生理特性和叶际细菌群落的影响

Effects of pesticide application and plant sexual identity on leaf physiological traits and phyllosphere bacterial communities

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